A micro-quantitative method for direct detection of bacterial endotoxin by fluorescence labelling of l-glycero-d-manno-heptose
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چکیده
منابع مشابه
Functional analysis of the glycero-manno-heptose 7-phosphate kinase domain from the bifunctional HldE protein, which is involved in ADP-L-glycero-D-manno-heptose biosynthesis.
The core oligosaccharide component of the lipopolysaccharide can be subdivided into inner and outer core regions. In Escherichia coli, the inner core consists of two 3-deoxy-d-manno-octulosonic acid and three glycero-manno-heptose residues. The HldE protein participates in the biosynthesis of ADP-glycero-manno-heptose precursors used in the assembly of the inner core. HldE comprises two functio...
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tion was reported earlier in the first demonstration of a microarray fabrication strategy based on noncovalent fluorous interactions. Mannose and other monosaccharides tagged by single C8F17 chains were noncovalently immobilized to fluorocarbon-coated glass slide surfaces and shown to tolerate conditions necessary for identifying binding partners such as conA. More recently, this fluorous-based...
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Lipooligosaccharide (LOS) is a critical virulence factor of Neisseria meningitidis. A Tn916 insertion mutant, designated 469, was found to exhibit a markedly truncated LOS of 2.9 kDa when compared by Tricine/SDS-PAGE to the parental LOS (4.6 kDa). Electrospray mass spectrometry analysis of 469 LOS revealed that it consisted of the deep rough, heptose-deficient structure, Kdo(2)-lipid A. Sequenc...
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glycero-manno-Heptose is widely present in the lipopolysaccharide (LPS) of most Gram-negative bacteria. This carbohydrate and its derivatives are sometimes also found in capsules and O antigens as well as in the glycan moieties of bacterial cell surface (S-layer) glycoproteins. Gram-negative bacteria with a heptoseless LPS are highly sensitive to hydrophobic substances and they also display red...
متن کاملThe crystal structure of the Y140F mutant of ADP-l-glycero-d-manno-heptose 6-epimerase bound to ADP-β-d-mannose suggests a one base mechanism
Bacteria synthesize a wide array of unusual carbohydrate molecules, which they use in a variety of ways. The carbohydrate L-glycero-D-manno-heptose is an important component of lipopolysaccharide and is synthesized in a complex series of enzymatic steps. One step involves the epimerization at the C6'' position converting ADP-D-glycero-D-manno-heptose into ADP-L-glycero-D-manno-heptose. The enzy...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1993
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1993.tb06446.x